Nitric oxide (NO) generated by neutrophils (PMN) is involved in tissue damage in the course of respiratory inflammation. However, little is known about participation of IL-8 in NO mediated lung injury in the course of Bovine Respiratory Disease (BRD). The purpose of this study was to evaluate how IL-8 influenced the generation of NO by neutrophils isolated from heifers in the course of acute and chronic BRD. Neutrophils isolated from blood were incubated with 0–1000 ng/ml of IL-8 and NO level was determined by Griess reaction after 0.5–72 h of incubation. Generation of NO increased with the growing concentration of IL-8. This augmentation was the greatest in acute BRD, less intense in chronic BRD and the least in healthy heifers. Both in healthy heifers and in both BRD groups, the maximal production of NO was observed in the presence of 1000 ng/ml IL-8. This observation revealed that generation of NO by neutrophils increased in dependence on the concentration of IL-8, time of incubation and group of animals. Therefore, augmented production of NO by neutrophils from heifers with BRD may lead to lung injury and worsening the course of disease.