The field experiment was conducted in the years 2009-2010. The aim of the study was to determine the effect of flat covering of plants with nonwoven fabric (PP17 and PP50) and inter-cropping on macronutrient content in the leaves of romaine lettuce ( Lactuca sativa L. var. romana Garst.). Covering the plants with PP17 and PP50 did not significantly affect the content of dry matter, total N, P and Ca in lettuce. Lettuce growing in flat covering accumulated less K and more Mg than that grown without nonwoven fabric. Lettuce growing with radishes accumulated the least of total N and the most of Ca in leaves. External leaves contained more dry matter, K, Ca, Mg while inner leaves were richer in total N and P.