Extraction methods, LC-ESI-MS/MS analysis of phenolic compounds and antiradical properties of functional food enriched with elderberry flowers or fruits

Abstrakt

Due to its pharmacological activity,Sambucus nigraL. is excellent potential componentof functional food. The main aim of presented study was the quantitative and qualitative analysis(LC-ESI-MS/MS) of phenolic acids and flavonoids present in extracts obtained from corn snackswith addition of elderberry fruits or flowers. Additionally, antiradical activity of samples wasdetermined. Moreover, the comparison of various extraction methods of polyphenols from thatfunctional food was made. In corn snacks, without additives, nine phenolic compounds were iden-tified. These were protocatechuic, 4-OH-benzoic, caffeic,p-coumaric, salicylic, ferulic acids, rutin,isoquercetin and apigenin-7-glucoside. In the extract fromSambucusflowers and extract fromsnacks containing 20% ofSambucusflowers fifteen polyphenols were found. They were additionallygallic, gentisic, vanillic, sinapic acids, kaempferol-3-rutinoside and astragalin. The radical-scavenging activity of the extracts was determined spectrophotometrically against DPPH(2,2-diphenyl-1-picrylhydrazyl) radical and using thin layer chromatography. The high antiradicalpotential was observed for crude extracts fromSambucusflowers or fruits and for extracts enrichedwith 20%, 10%, 5% ofSambucusflowers. The product with a 20% addition of fruits revealed mod-erate free radical scavenging properties

Autorzy

Marta Olech
Marta Olech
Karolina Wojtunik-Kulesza
Karolina Wojtunik-Kulesza
artykuł
Arabian Journal of Chemistry
Angielski
2019
12
8
4719-4730
otwarte czasopismo
CC BY-NC-ND Uznanie autorstwa-Użycie niekomercyjne-Bez utworów zależnych 4.0
ostateczna wersja opublikowana
w momencie opublikowania
2019-12-01
70
4,762
39
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