Nickel in excessive amounts deteriorates the nutritional status in plants, inter alia reduces the level of sulphur. Simultaneously, the common use of NPK fertilizers without sulphates (S-SO4) together with the progressive process of reducing emissions of S compounds to the natural environment may lead to deficiency of this element involved in stress tolerance. The aim of the studies was to evaluate the changes in the macronutrient contents and accumulation in Sinapis alba L. cv. Rota grown for 2 weeks at different levels of S-SO4 (S-2-standard dose, 6, 9 mM) and Ni (0, 0.0004, 0.04, 0.08 mM) in modified Hoagland’s nutrient solution. Intensive S-SO4 nutrition of Ni-exposed mustard seems to improve to some extent the unfavourable macronutrient status. It substantially increased the root macronutrient content drop in the content of this macronutrient. The macronutrient status in shoots remained stable, except for the significant increase in the S content and the rise in Ca accumulation accompanied by an increase in N and K accumulation recorded at the S dose 6 mM. Ni-bioaccumulation did not change markedly with the exception of its increase in roots at the 6 mM S level.