The purpose of this research was to determine the behavioural reactivity and the heart rate of Konik polski horses during routine handling activities in the stable. The study included 20 horses ofKonik polski breed (12 mares and 8 stallions) kept in the Centre for Conservation Breed-ing of Konik polski horses in Florianka. The horse’s behaviour was assessed on a scale of 1 point (aversive behaviour) to 5 points (affiliative behaviour) and the heart rate was telemetrically re-corded during particular stages of handling (approach to the horse, brushing, bridling, legs rising) in the stable. Significant negative correlationsbetween the animal’s behaviour scores and their heart rate values were noticed. The mares were ch aracterised by a higher heart rate than the stal-lions. The horses whose behaviourwas assessed positively during the handler’s approach were also characterised by affiliative behaviour during bridling