Characterization of active compounds of different garlic (Allium sativum L.) cultivars

Abstrakt

Garlic (Allium sativum L.) has a reputation as a therapeutic agent for many different diseases such as microbial infections, hypertension, hypercholesterolaemia, diabetes, atherosclerosis and cancer. Health benefits of garlic depend on its amount of biologically active compounds, which differs between cultivars and geographical regions. The aim of this study was to evaluate and compare the biological activity of aqueous extracts from nine garlic varieties from different countries (Poland, Spain, China, Portugal, Burma, Thailand and Uzbekistan). Antioxidant properties were evaluated through free radical scavenging (DPPH•, ABTS•+) and ion chelation (Fe2+, Cu2+) activities. The cytotoxicity of garlic extracts was evaluated in vitro using Neutral Red Uptake assay in normal human skin fibroblasts. The obtained results revealed that garlic extracts contained the highest amount of syringic and p-hydroxybenzoic acids derivates. The lowest IC50 values for DPPH•, ABTS•+ scavenging and Cu2+ chelation ability was determined in Chinese garlic extracts (4.63, 0.043 and 14.90 µg/mL). Extract from Spanish cultivar Morado and Chinese garlic were highly cytotoxic for human skin fibroblasts as they reduced cellular proliferation by 70-90%. We show diverse contents of proteins and phenolic components in garlic bulbs from different varieties. The obtained results could help to choose the varieties of garlic which contain significant amounts of active compounds, have important antioxidant properties and display low antiproliferative effect and/or low cytotoxicity against normal human skin fibroblast BJ.

Autorzy

Konrad. A. Szychowski
Konrad. A. Szychowski
Katarzyna Gaweł-Bęben
Katarzyna Gaweł-Bęben
Magdalena Matysiak
Magdalena Matysiak
Urszula E. Binduga
Urszula E. Binduga
Jan Gmiński
Jan Gmiński
artykuł
POLISH JOURNAL OF FOOD AND NUTRITION SCIENCES
Angielski
2018
68
1
73-81
otwarte czasopismo
CC BY-NC-ND Uznanie autorstwa-Użycie niekomercyjne-Bez utworów zależnych 4.0
ostateczna wersja opublikowana
w momencie opublikowania
2018-03-31
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1,514
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