The results of the research concerned the effect of seed stimulation with He-Ne laser light on the content of photosynthetic pigment in scorzonera leaves of the Maxima cultivar. It was also examined whether the exposure of the seeds could affect the lifetime of chlorophyll a fluorescence of scorzonera leaves extract and scorzonera leaves. The research material were leaves which came from plants grown from seeds harvested in 2009, 2010, 2011 and 2012. The germination capacity of the seeds was 50.8, 71.0, 93.0 and 79.3%. Prior to sowing, the seeds were subjected to electromagnetic stimulation with He-Ne laser light with a wavelength of 632.8 nm and a surface power density of 3 mW cm–2 for 1, 5, 10 and 30 minutes. Untreated seeds were the control. The content of chlorophyll a and b and carotenoids in leaves was higher in plants grown from younger seeds (harvested in 2011 and 2012) than from older seeds (harvested in 2009 and 2010). Stimulation of younger seeds with laser light caused a reduction of the content of these pigments. The lifetime of chlorophyll a fluorescence of the leaves extract was longer than that of the leaves. There was no significant effect of laser stimulation of seeds on chlorophyll a fluorescence lifetime in leaves extract of scorzonera. This parameter was higher in leaves extract of plants grown from younger seeds (harvested in 2011, 2012) than from older seeds (harvested in 2009, 2010).