The aim of the study was to evaluate the effect of crop protection and seeding ratio on the contents of macronutrients (nitrogen, phosphorus, potassium, calcium, magnesium) in grain of two spelt cultivars: Polish ‘Rokosz’ and German ‘Schwabenspelz’. The field experiment was conducted in Czesławice in 2014–2015 on lessive soil developed from loess classified as good wheat complex and II bonitation class, slightly acidic. The plowing layer of the soil was characterized by a high content of available forms of phosphorus, potassium and average magnesium. Material for analysis consisted of winter spelt grain. The first experimental factor: winter spelt varieties; second factor: two seeding ratios; third factor: crop protection. The harvest was performed at full grain maturity stage. Content of three basic macronutrients in spelt grain: nitrogen, phosphorus and potassium, was varied for cultivars. Meanwhile two cultivars did not differ with the amount of magnesium and calcium in grain. ‘Schwabenspelz’ was more abundant than ‘Rokosz’ regarding to studied macronutrients. The increase of plant density resulted in significant reduction in the nitrogen content and increase in the amount of phosphorus in winter spelt grain. The amount of plant density did not affect the abundance of spelt grain in relation to metals included in macronutrients occurring at plants. The crop protection treatments of winter spelt did not impact on the macronutrients content in grain.