The field experiment was conducted to evaluate how row intercropping of the lentil with naked oats as a supporting plant impacts macro- and micronutrient content of two lentil varieties ‘Tina’ and ‘Anita’ as well as a mixture of these two varieties. Additionally, amino acid content and fatty acid composition of lentil seeds were determined. Lentil was sown at a row spacing of 20 and 25 cm. In var. ‘Tina’ seeds, a higher content of K, Ca, Mg, Mn and B was determined than in var. ‘Anita’. ‘Anita’ seeds, in turn, were characterized by a higher content of fat, N, P, Cu, Zn and Fe. Lentil seeds sown at a spacing of 20 cm had a higher content of P, K, Ca, Cu, Zn and B compared to a spacing of 25 cm, but they contained less nitrogen. The presence of oats as a supporting crop deteriorated the quality of lentil seeds by contributing to a reduced content of N, P, K, Cu, Zn, Mn, Fe and B compared to sole cropped lentil. A higher content of C18:0, C18:2, C18:3, C20:0, OMEGA 3, and OMEGA 6 acids was determined in ‘Anita’ seeds. Var. ‘Tina’, in turn, contained more C16:0, C18:1 acids. In ‘Anita’ seeds, the content of most evaluated amino acids was determined to be significantly higher than in ‘Tina’ seeds.