The aim of the study was to determine the influence of pumpkin flour addition and storage time on mechanical properties of yeast dough. A control sample was prepared for the tests, and 3 cakes with pumpkin flour addition in the amount of 5, 10 and 15% in the mixture. The cakes were stored for 4 days. The scope of work included determination of the moisture and acidity and of the mechanical properties of cake according to the TPA method (Texture Profile Analysis). The yeast cake with pumpkin flour addition was subjected to sensory evaluation. The study showed that both the addition of pumpkin flour and storage time had a significant effect on the mechanical properties of the texture. It was shown that increased storage time and pumpkin flour addition increased the hardness and chewiness of cakes. The highest values hardness (66 N) and chewiness (11.30 N) were noted for cakes with 15% addition of pumpkin flour on the fourth day of storage time. Springiness and cohesiveness decreased with increasing level of pumpkin flour addition. The cake with 15% pumpkin flour addition, on the fourth day of storage, was characterised by the lowest value of springiness (0.61) and cohesiveness (0.24). The results of sensory analysis of the cakes showed that cakes with 15% pumpkin flour addition had the best sensory properties and qualified for the first level of quality.