Despite the many studies on Wolbachia in Coleoptera, data about the prevalence of this bacterium among ground and rove beetles are missing. This study describes Wolbachia distribution and diversity in predatory beetles co-habiting the same environment - submontane river channels. Three species of Paederini (Staphylinidae) and four Bembidiini (Carabidae) were collected from six river catchments of the Carpathians. Wolbachia was absent in three species: Paederidus rubrothoracicus (Goeze), Bembidion decorum (Zenker in Panzer), Bembidion modestum (F.) - but detected in four others. Paederus limnophilus Erichson and Paederidus ruficollis F. were infected at only some sites by different strains of supergroup A, Bembidion punctulatum Drapiez was infected at all sites by a single strain from supergroup B, and Bembidion varicolor F. was single or double infection by three strains from A and B supergroups. The patterns of infection prevalence and strain diversity were very complex and only in some cases support common Wolbachia infection and strain similarity among different species of predatory beetles sharing the same environment. Moreover, the similar genetic patterns observed in all examined beetles cannot be associated with the infection status of particular species (distinct mitochondrial and nuclear haplotypes fixed in distant populations; small diversity within populations).