Structural changes in the enamel surface subjected to induced demineralization and assessment of the influenceof prenatal administration ofβ-hydroxyβ-methylbutyrate (HMB) on enamel resistance were investigated. Theexamination was conducted onfive sets of teeth from one-day-old spiny mice (Acomys cahirinus), one fromthe control and four from the experimental groups. Surface structure, molecular arrangement and crystalline or-ganization of offspring's enamel both before and after etching were studied. Obtained results revealed that thephysical and molecular arrangements of enamel were altered after the prenatal supplementation, and signifi-cantly affected itsfinal structure and resistance against acid action. The enamel of incisors from the offspringwhich mothers were supplemented with HMB in a high dose (0.2 g/kgbw) and in the late period of gestation(26th–39th day) showed the highest endurance against acid treatment demonstrating only vestigial changesin their surface structure after acid action. Comparing to the remaining experimental groups, it was characterizedby a reduced roughness and fractal dimension, significantly lower degree of demineralization and simultaneouslack of notable differences in the Raman spectra before and after acid etching. The results suggest that an in-creased enamel resiliency was the effect of a relatively high degree of mineralization and higher organizationof the surface