According to the assumptions of Organisation for Economic Co-operation and Development OECD, the share of biofuels in the global transport sector is estimated to reach 15–23% by 2050. The triticale can be used to produce bioethanol. The appropriate production process should generate as much renewable energy as possible per production unit. Plant production can be carried out in various tillage systems and using appropriate doses of nitrogen fertilization. The objective of this study is to compare the effect of traditional tillage system (TRD) and reduced (RED) tillage technology and nitrogen fertilizer (0, 40, 80, 120 kg N ha−1) on grain and bioethanol yield of spring triticale. The field experiment was performed in the south east of Poland (50°42′ N, 23°15′ E) on medium dystrophic typical brown soil. Based on research and calculations, the TRD system and between 40 and 80 kg ha−1 of N fertilizer are recommended for use in the cultivation of triticale for bioethanol production purposes. Such a variant will ensure a sufficient yield of grain (5.190 and 5.803 t ha−1), starch (3.462 and 3.871 t ha−1) and bioethanol (2487.3 and 2780.7 L ha−1) and good agronomic efficiency of N fertilizer (16.96 and 12.15 L of bioethanol per 1 kg of nitrogen (N) applied). The best ratio of energy efficiency of bioethanol production (EROI—Energy Return on (Energy) Investment or “net energy”) was recorded for the TRD system (1.138:1) and for the N fertilizer at 40 kg N ha−1 (1.144:1)