Imidacloprid markedly affects hemolymph proteolysis, biomarkers, DNA global methylation, and the cuticle proteolytic layer in western honeybees

Abstrakt

Imidacloprid (IMD) may affect proteolysis, aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphatase (ALP), and global DNA methylation in honeybees. Queens, drones, and workers aged 1 or 20 days were exposed (free-flying colonies) to IMD (5 ppb and 200 ppb) in their diet. As a result, the colony depopulation did not occurred. IMD disturbed hemolymph/cuticle proteolysis; deactivated most of the cuticle protease inhibitors, activated hemolymph thiol and metal proteases and cuticle thiol proteases; downregulated ALP, ALT, AST; and increased DNA methylation in a caste- and age-dependent manner. The response in queens and workers differed, possibly due to eusocial evolution. Higher IMD dose had greater effects. The responses of ALP, ALT, AST, and DNA may reflect acceleration of biochemical senescence and epigenetic adaptation to IMD. All these biochemical side effects may lead to colony depopulation during future biotic/abiotic stress.

Autorzy

Jerzy Wilde
Jerzy Wilde
Maciej Siuda
Maciej Siuda
Beata Bąk
Beata Bąk
artykuł
APIDOLOGIE
Angielski
2020
51
620-630
otwarte czasopismo
CC BY 4.0 Uznanie autorstwa 4.0
ostateczna wersja opublikowana
w momencie opublikowania
2020-03-16
140
2,318
12
19