The aim of the study was to identify the trends and dynamics of changes in the chemical and biochemical properties of soil induced by rotational grazing of farmed deer. Soils pH and the content of mineral nitrogen forms, total nitrogen, and organic carbon were determined in the study and the activity of three soil enzymes. The monitored soils were slightly acidic and neutral, with pH in 1 mol KCldm−3 ranging from 6.37 to 7.03. The contents of content of organic carbon, total nitrogen in the monitored soils were in the range of 9.42–19.37 gkg−1 and 1.13–2.24 gkg−1, respectively, while the contents of N-NH4+ and N-NO3− of 1.34–7.08 mgkg−1 and 102.8–619.5 mgkg−1, respectively. The mean levels of nitrate nitrogen in the pasture soils were lower than that in the mown soil. The soils from the pastures were characterized by a higher level of available phosphorus, potassium, and magnesium forms than the mown soil. In the deer grazing areas, the activity of all enzymes was clearly higher than in the soil from the control plot. The results of the present pilot study have demonstrated a positive effect of cervid grazing on the biochemical parameters of the soil environment