Background:Breed specific reference ranges for selected blood parameters are recommended for proper interpretation ofblood tests, but there are only few reports dealing with ponies. The purpose of this study was to investigate if bloodparameters differ among ponies’classes and to check if generalnormal values for equine species are applicable to ponies.Results:All, except total protein concentration,biochemical parameter significantly (p< 0.05) differed among ponies’classes.The most pronounced difference was noted in blood lactate concentrations, higher (p< 0.001) in the smallest ponies (classA). In all groups of ponies muscle enzymes (aspartate aminotransferase and creatine kinase) and urea were high whencompared to normal values for equine species, but triglycerides and creatinine were low. Blood lactate concentration washigh in comparison with normal values for horses only in class A ponies’.Conclusions:In healthy ponies, blood lactate concentration significantly differs between height classes. Normal values forequine species should not be directly applied to interpret the lactate, triglycerides, aspartate aminotransferase and creatinekinase values in ponies.