Livestock buildings are often contaminated with bacterial and fungal microflora. Animals living in the buildings, especially their excreta and secretions and their feed, can be a source of microor-ganisms, including pathogens. Significant microbial contamination occurs in pig houses, poultry houses and cowsheds. The microbes most frequently isolated from the air of these buildings are bacteria of the genera Streptococcus, Staphylococcus, Bacillus, and Clostridium and of the fam-ily Enterobacteriaceae. Among fungi, the most common are Aspergillus, Trichoderma, Penicillium, Cladosporium and Alternaria. Microbes present in livestock buildings often pose a hazard to work-ers, in whom they can cause infectious and allergic diseases, especially respiratory disease. Bacte-rial endotoxins may also pose a threat to humans and animals. For this reason it is important to carry out microbiological monitoring and preventive measures on livestock farms and to maintain appropriate environmental conditions. This will reduce microbiological contamination of livestock buildings and improve both workers’ health and animal welfare.