Profiling glioblastoma cases with an expression of DCX, OLIG2 and NES

Abstrakt

Glioblastoma (GBM) remains the leading cause of cancer-related deaths with the lowest five-year survival rates among all of the human cancers. Multiple factors contribute to its poor outcome, including intratumor heterogeneity, along with migratory and invasive capacities of tumour cells. Over the last several years Doublecortin (DCX) has been one of the debatable factors influencing GBM cells’ migration. To resolve DCX’s ambiguous role in GBM cells’ migration, we set to analyse the expression patterns of DCX along with Nestin (NES) and Oligodendrocyte lineage transcription factor 2 (OLIG2) in 17 cases of GBM, using immunohistochemistry, followed by an analysis of single-cell RNA-seq data. Our results showed that only a small subset of DCX positive (DCX+) cells was present in the tumour. Moreover, no particular pattern emerged when analysing DCX+ cells relative position to the tumour margin. By looking into single-cell RNA-seq data, the majority of DCX+ cells were classified as non-cancerous, with a small subset of cells that could be regarded as glioma stem cells. In conclusion, our findings support the notion that glioma cells express DCX; however, there is no clear evidence to prove that DCX participates in GBM cell migration. View Full-Text

Autorzy

Adrian Odrzywolski
Adrian Odrzywolski
Bożena Jarosz
Bożena Jarosz
Michał Kiełbus
Michał Kiełbus
Ilona Tolejko
Ilona Tolejko
Dominik Ziemianek
Dominik Ziemianek
Radosław Rola
Radosław Rola
artykuł
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
Angielski
2021
22
24
13217
otwarte czasopismo
CC BY 4.0 Uznanie autorstwa 4.0
ostateczna wersja opublikowana
w momencie opublikowania
2021-12-08
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6,208
0
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