Tonic immobility (TI) is a phenomenon known as thanatosis or apparent death. The phenomenon can be induced in many ways. It occurs, for example, in the presence of a hazard, as a result of coercion through an unnatural body position or as a result of administration of certain medicines. TI is sometimes used in veterinary practice to tame patients. The aim of the study was to determine the effect of tonic immobility induction on selected physiological parameters in Oryctolagus cuniculus f. Domesticus rabbits. The study included 20 healthy rabbits, patients of a veterinary clinic, divided into two groups of 10 individuals. In the study group, TI was induced to conduct a standard clinical trial, while the control group were subjected to a standard clinical trial without TI. Heart rate, respiratory rate, pupil diameter and blood glucose concentration were measured in all animals. The measurements were performed three times: after the patient’s arrival at the clinic, after a detailed clinical examination in a normal or tonic immobility position and 15 minutes after the end of the examination. Additionally, the blood cortisol concentration was measured twice: on arrival at the clinic and at the end of the clinical trial. The results of the experiment show that miniature rabbits feel less stress during tonic immobility testing than rabbits in the standing position, and therefore this phenomenon should be used for taming animals.