Hesperidin (HE), chrysin (CH) and naringenin (NA) are flavonoids, being the most important group of polyphenols, and show anti-inflammatory properties which have been demonstrated on various models. Polyphenols have a lot of biological properties, such as antioxidative, antiviral, immunomodulatory and anticancer activities. However, the effect on mastitis has not been yet described. This research aimed to analyse the tolerability of selected polyphenols after multiple intramammary administrations (IMM) as well as to investigate their potential impact on somatic cell count (SCC) in mastitis dairy cows. The study was performed on 12 Polish Holstein Black-and-White cows in their 4th to 6th lactation. Only animals with inflammation in one-quarter of the udder were selected. The selection was based on SCC and clinical assessment. The experiment was performed with multiple intramammary administrations with each of these polyphenols in dairy cows affected with mastitis. Polyphenols were administered at a dose of 30 mg/quarter/day. Milk samples for SCC, blood plasma samples for pharmacokinetics and blood haematology and biochemistry (selected blood parameters were tested) were collected at baseline, treatment period and within the recovery period. Positive effects concerning the SCC in milk of mastitic cows were confirmed for all tested polyphenols.