The manuscript describes the results of an experimental study of the level of PM (particulate matter) emissions arising from the combustion of two selected types of biomass (i.e., rapeseed straw pellets and engine biofuel (biodiesel, FAME)), which were derived from rapeseed. The PM emissions from the combustion of biofuels were compared with those obtained from the combustion of their traditional counterparts (i.e., wood pellets and diesel fuel). Both types of pellets were burned in a 10 kW boiler designed to burn these types of fuels. The engine fuels tested were burned in a John Deere 4045TF285JD engine mounted on a dynamometer bench in an engine dyno, under various speed and load conditions. A Testo 380 analyzer was used to measure the PM emission levels in boiler tests, while an MPM4 particle emission meter was used in the engine tests. The combustion (under rated conditions) of rapeseed straw pellets resulted in a significant increase in PM emissions compared to the combustion of wood pellets. The PM emissions during the combustion of wood pellets were 15.45 mg·kg−1, during the combustion of rapeseed straw pellets, they were 336 mg·kg−1, and the calculated emission factors were 44.5 mg·MJ−1 and 1589 mg·MJ−1, respectively. In the engine tests, however, significantly lower particulate emissions were obtained for the evaluated biofuel compared to its conventional counterpart. The combustion of rapeseed oil methyl esters resulted in a 40–60% reduction in PM content in the exhaust gas on average for the realized engine speeds over the full load range compared to the combustion of diesel fuel.