Synergistic antifungal interactions between antibiotic amphotericin b and selected 1,3,4-thiadiazole derivatives, determined by microbiological, cytochemical, and molecular spectroscopic studies.

Abstrakt

In recent years, drug-resistant and multidrug-resistant fungal strains have been more frequently isolated in clinical practice. This phenomenon is responsible for difficulties in the treatment of infections. Therefore, the development of new antifungal drugs is an extremely important challenge. Combinations of selected 1,3,4-thiadiazole derivatives with amphotericin B showing strong synergic antifungal interactions are promising candidates for such formulas. In the study, microbiological, cytochemical, and molecular spectroscopy methods were used to investigate the antifungal synergy mechanisms associated with the aforementioned combinations. The present results indicate that two derivatives, i.e., C1 and NTBD, demonstrate strong synergistic interactions with AmB against some Candida species. The ATR-FTIR analysis showed that yeasts treated with the C1 + AmB and NTBD + AmB compositions, compared with those treated with single compounds, exhibited more pronounced abnormalities in the biomolecular content, suggesting that the main mechanism of the synergistic antifungal activity of the compounds is related to a disturbance in cell wall integrity. The analysis of the electron absorption and fluorescence spectra revealed that the biophysical mechanism underlying the observed synergy is associated with disaggregation of AmB molecules induced by the 1,3,4-thiadiazole derivatives. Such observations suggest the possibility of the successful application of thiadiazole derivatives combined with AmB in the therapy of fungal infections

Autorzy

Agnieszka Dróżdż
Agnieszka Dróżdż
Dominika Kubera
Dominika Kubera
Adrianna Sławińska-Brych
Adrianna Sławińska-Brych
Lidia Ślusarczyk
Lidia Ślusarczyk
Dariusz Karcz
Dariusz Karcz
Alina Olender
Alina Olender
Agnieszka Bogut
Agnieszka Bogut
Daniel Pietrzak
Daniel Pietrzak
Wojciech Dąbrowski
Wojciech Dąbrowski
Andrzej Stepulak
Andrzej Stepulak
Alicja Wójcik-Załuska
Alicja Wójcik-Załuska
artykuł
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
Angielski
2023
24
4
3430
otwarte czasopismo
CC BY 4.0 Uznanie autorstwa 4.0
ostateczna wersja opublikowana
w momencie opublikowania
2023-02-08
140
4,9
0
0