Response of murine gut microbiota to a prebiotic based on oligosaccharides derived via hydrolysis of fungal α-(1→3)-d-glucan: preclinical trial study on mice

Abstrakt

We investigated the modulating effect of α-(1→3)-glucooligosaccharides (GOS), i.e. a product of fungal α-(1→3)-d-glucan hydrolysis, on the gut microbiota composition. Mice were fed with a GOS-supplemented diet and two control diets for 21 days, and fecal samples were collected at 0, 1, and 3-week time points. The bacterial community composition was determined by 16S rRNA gene Illumina sequencing. The gut microbiota of the GOS-supplemented mice showed profound time-dependent changes in the taxonomic composition; however, we did not observe significant changes in α-diversity indices. The biggest number of genus abundance shifts after 1 week of the treatment was noticed between the group of the GOS-supplemented mice and the controls; however, the differences were still relevant after the 3-week treatment. The GOS-supplemented mice displayed higher abundance of Prevotella spp., with a concomitant decrease in the abundance of Escherichia-Shigella. Hence, GOS seems to be a promising candidate for a new prebiotic.

Autorzy

Ewa Sajnaga
Ewa Sajnaga
Katarzyna Socała
Katarzyna Socała
Agnieszka Kalwasińska
Agnieszka Kalwasińska
Piotr Wlaź
Piotr Wlaź
Monika Elżbieta Jach
Monika Elżbieta Jach
Michał Tomczyk
Michał Tomczyk
Adrian Wiater
Adrian Wiater
artykuł
FOOD CHEMISTRY
Angielski
2023
417
135928
200
8,5
0
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