Repetitive cerulein-induced chronic pancreatitis in growing pigs - a pilot study

Abstrakt

Chronic pancreatitis (CP) is an irreversible and progressive inflammatory disease. Knowledge on the development and progression of CP is limited. The goal of the study was to define the serum profile of pro-inflammatory cytokines and the cell antioxidant defense system (superoxidase dismutase—SOD, and reduced glutathione—GSH) over time in a cerulein-induced CP model and explore the impact of these changes on selected cytokines in the intestinal mucosa and pancreatic tissue, as well as on selected serum biochemical parameters. The mRNA expression of CLDN1 and CDH1 genes, and levels of Claudin-1 and E-cadherin, proteins of gut barrier, in the intestinal mucosa were determined via western blot analysis. The study showed moderate pathomorphological changes in the pigs’ pancreas 43 days after the last cerulein injection. Blood serum levels of interleukin (IL)-1-beta, IL-6, tumor necrosis factor alpha (TNF-alpha), C-reactive protein (CRP), lactate dehydrogenase (LDH), gamma-glutamyl transpeptidase (GGTP), SOD and GSH were increased following cerulein injections. IL-1-beta, IL-6, TNF-alpha and GSH were also increased in jejunal mucosa and pancreatic tissue. In duodenum, decreased mRNA expression of CDH1 and level of E-cadherin and increased D-lactate, an indicator of leaky gut, indicating an inflammatory state, were observed. Based on the current results, we can conclude that repetitive cerulein injections in growing pigs not only led to CP over time, but also induced inflammation in the intestine. As a result of the inflammation, the intestinal barrier was impaired.

Autorzy

Małgorzata Świątkiewicz
Małgorzata Świątkiewicz
Janine Donaldson
Janine Donaldson
Katarzyna Ropka-Molik
Katarzyna Ropka-Molik
Maciej Murawski
Maciej Murawski
Tomasz Schwarz
Tomasz Schwarz
Piotr Dobrowolski
Piotr Dobrowolski
Sławomir Dresler
Sławomir Dresler
Joanna Bonior
Joanna Bonior
artykuł
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
Angielski
2023
24
9
7715
otwarte czasopismo
CC BY 4.0 Uznanie autorstwa 4.0
ostateczna wersja opublikowana
w momencie opublikowania
2023-04-23
140
4,9
0
1