Antioxidant and antimicrobial properties of an extract rich in proteins obtained from Trametes versicolor

Abstrakt

Introduction: Bioactive proteins and peptides generated from fruit, vegetables, meat or fish have great potential as functionalfood or substitutes for antibiotics. In recent years it has also been demonstrated that the fungus kingdom could be a source of thesecompounds. The study investigated the bioactivity of an extract of the lignicolous fungus Trametes versicolor and its hydrolysate.Material and Methods: The fungus was collected in a mixed forest in October, extracted and hydrolysed. To inspect the proteinand peptide profiles before and after hydrolysis, matrix-assisted laser desorption/ionisation–time-of-flight mass spectrometricanalysis was performed. To evaluate the antioxidant properties of the preparations, 2,2-diphenyl-1-picrylhydrazyl (DPPH●) and2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid (ABTS●+) radical scavenging assays were used. The activity of the fungusextract and hydrolysate against Aeromonas veronii, Bacillus cereus, Enterococcus faecalis, Enterococcus faecium, Escherichiacoli, Klebsiella pneumoniae, Pseudomonas aeruginosa, Salmonella Typhimurium, Staphylococcus aureus, Staphylococcusepidermidis, Streptococcus agalactiae, Streptococcus dysgalactiae, and Streptococcus uberis was determined by the minimuminhibitory concentration and minimum bactericidal concentration values. Results: The extract and its hydrolysate showed almost100% ABTS●+ and DPPH● radical scavenging with a low half maximal inhibitory concentration. The water extract and hydrolysateof T. versicolor exhibited antimicrobial activity against two S. aureus strains, E. coli, P. aeruginosa and Salmonella Typhimurium.Conclusion: These results provide compelling evidence that the analysed fungus extract and its hydrolysate hold promise withtheir antibacterial and antioxidant properties.

Autorzy

Kosikowska Urszula
Kosikowska Urszula
Andrzejczuk Sylwia
Andrzejczuk Sylwia
Garbacz Katarzyna
Garbacz Katarzyna
Anna Dobrut
Anna Dobrut
Wiktoria Czupryna
Wiktoria Czupryna
artykuł
Journal of Veterinary Research
Angielski
2023
67
2
209-218
otwarte czasopismo
CC BY-NC-ND Uznanie autorstwa-Użycie niekomercyjne-Bez utworów zależnych 4.0
ostateczna wersja opublikowana
w momencie opublikowania
2023-06-12
200
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