Agricultural chambers together with agricultural advisory centers and other entities are part of the European Union Agricultural Knowledge and Innovation System (AKIS). The system consists of a network of entities whose activities are focused on the introduction of new products, technologies, and organizational forms for use in agriculture. The AKIS is also defined as a network of research institutes, advisory centers, agricultural chambers, agricultural schools, and other non- governmental organizations that help farmers to upgrade the innovativeness and competitiveness of their farms and solve agriculture-related problems. In Poland, the activities of self-government in the agricultural sector are specified by the Act on Agricultural Chambers of 14 December 1995. An important research problem is the assessment of the activities of agricultural chambers in terms of the transfer of knowledge and innovations in agriculture in Poland and the determination of their place in the AKIS. This study of the impact of agricultural self-government on the transfer of knowledge and innovations in agriculture was carried out through the analysis of selected agricultural chambers in various regions of Poland taking into account the differences in the average surface area of agricultural land belonging to one farm. The research shows that agricultural chambers’ activities had a substantial impact on the rate of absorption of EU funds under the Rural Development Program in the analyzed regions. The comparison of the effectiveness of the agricultural chambers in Poland with the activities of chambers in the other EU Member States indicates a weak influence of the former on developmental processes in agriculture. Agricultural chambers should actively participate in the formulation of the national agricultural policy, take greater measures to ensure the stability of agricultural income and the flow of information, and be an important partner for farmers in representing their interests.