Bacteria of the genus Staphylococcus are a component of the natural microbiota of the skin and mucous membranes of animals and humans, but they can also cause endogenous or exogenous infections. They are divided into two groups: coagulase-positive staphylococci (CoPS), which have the ability to clot blood plasma, and coagulase-negative staphylococci (CoNS). The intraspecific diversity of CoPS strains also translates into the variety of features presented by individual strains. A significant problem in veterinary medicine and animal husbandry is the growing resistance of staphylococci to antibiotics. Due to the progress of civilisation, contact between wildlife and the human environment is becoming more frequent, increasing the possibility of the exchange of microbial virulence factors in various ecosystems. The aim of the study was to analyse the occurrence and assessment of the pathogenic potential of individual species of coagulase-positive staphylococci isolated from dead capercaillies kept in aviaries as well as free-living birds in Southeastern Poland. The occurrence of multidrug-resistant CoPS in aviary capercaillies suggests their role in the transmission and spread of resistant strains into the environment. Our results also suggest that free-living birds may be a significant reservoir of enterotoxigenic Staphylococcus strains.