Abstrakt
The aim of this study was to evaluate the antioxidative/oxidative status of spermatozoa and
prostatic fluid in dogs with benign prostatic hyperplasia (BPH) by the determination of total antioxidant capacity and protein peroxidation markers. Study was conducted on 40 intact dogs
of various breeds. The dogs were assigned to two groups: BPH group (n=20) and non-affected
group (n=20). The second and third fractions of the ejaculate were collected separately by digital
manipulation. Total antioxidant capacity (TAC) and the concentrations of SH-groups in sperm
and prostatic fluid were determined spectrophotometrically, the concentrations of bityrosine and
formylkynurenine were determined using spectrofluorimetric methods. The mean values of TAC
in spermatozoa and prostatic fluid were significantly lower (p<0.05), whereas the mean contents
of biotyrosine and formylkinurenine were significantly higher (p<0.05) in BPH dogs compared
to control dogs. There was no statistically significant difference in the content of SH group
between dogs with BPH and control dogs (p>0.05). In conclusion, the results indicate that BPH
in dogs is associated with reduced total antioxidant capacity and increased protein oxidation
in the prostatic fluid and spermatozoa, and suggest the importance of oxidative stress in the pathogenesis of this condition. The potential role of antioxidants in the prevention and therapy
of canine BPH requires further studies.