Does chronic pancreatitis in growing pigs lead to articular cartilage degradation and alterations in subchondral bone?

Abstrakt

Chronic pancreatitis (CP), a progressive inflammatory disease, poses diagnostic challenges due to its initially asymptomatic nature. While CP’s impact on exocrine and endocrine functions is well-recognized, its potential influence on other body systems, particularly in young individuals, remains underexplored. This study investigates the hypothesis that CP in growing pigs leads to alterations in articular cartilage and subchondral bone, potentially contributing to osteoarthritis (OA) development. Utilizing a pig model of cerulein-induced CP, we examined the structural and compositional changes in subchondral bone, articular cartilage, and synovial fluid. Histological analyses, including Picrosirius Red and Safranin-O staining, were employed alongside immuno-histochemistry and Western blotting techniques. Our findings reveal significant changes in the subchondral bone, including reduced bone volume and alterations in collagen fiber composition. Articular cartilage in CP pigs exhibited decreased proteoglycan content and alterations in key proteins such as MMP-13 and TGF-β1, indicative of early cartilage degradation. These changes suggest a link between CP and musculoskeletal alterations, underscoring the need for further research into CP’s systemic effects. Our study provides foundational insights into the relationship between CP and skeletal health, potentially guiding future pediatric healthcare strategies for early CP diagnosis and management.

Autorzy

Monika Hułas-Stasiak
Monika Hułas-Stasiak
Piotr Dobrowolski
Piotr Dobrowolski
Małgorzata Świątkiewicz
Małgorzata Świątkiewicz
Tomasz Blicharski
Tomasz Blicharski
Janine Donaldson
Janine Donaldson
Joanna Bonior
Joanna Bonior
artykuł
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
Angielski
2024
25
1989
otwarte czasopismo
CC BY 4.0 Uznanie autorstwa 4.0
ostateczna wersja opublikowana
w momencie opublikowania
2024-02-06
140
4,9
0
0