Characteristic of the gene candidate SecARS encoding alkylresorcinol synthase in Secale

Abstrakt

Background: Alkylresorcinols (ARs) are compounds belonging to the class of phenolic lipids. A rich source of ARs are cereal grains such as rye, wheat, triticale or barley. ARs found in plants are characterized by a variety of biological properties such as antimicrobial, antifungal and cytotoxic activity. Moreover, they are proven to have a positive influence on human health. Here, we aimed to find and characterize the gene with ARs synthase activity in the species Secale cereale. Methods and results: Using BAC library screening, two BAC clones containing the gene candidate were isolated and sequenced. Bioinformatic analyses of the resulting contigs were used to examine the structure and other features of the gene, including promoter, intron, 3’UTR and 5’UTR. Mapping using the FISH procedure located the gene on the 4R chromosome. Comparative analysis showed that the gene is highly similar to sequences coding for type III polyketide synthase. The level of gene expression in various parts of the plant was investigated, and the biochemical function of the gene was confirmed by heterologous expression in yeast. Conclusions: The conducted analyses contributed to a better understanding of the processes related to ARs synthesis. Although the research concerned the rye model, the knowledge gained may help in understanding the genetic basis of ARs biosynthesis in other species of the Poaceae family as well.

Autorzy

Małgorzata Targońska-Karasek
Małgorzata Targońska-Karasek
Michał Kwiatek
Michał Kwiatek
Jolanta Groszyk
Jolanta Groszyk
Jakub Walczewski
Jakub Walczewski
Mariusz Kowalczyk
Mariusz Kowalczyk
Sylwia Pawelec
Sylwia Pawelec
Maja Boczkowska
Maja Boczkowska
Anna Rucińska
Anna Rucińska
artykuł
MOLECULAR BIOLOGY REPORTS
Angielski
2023
50
8373–8383
otwarte czasopismo
CC BY 4.0 Uznanie autorstwa 4.0
ostateczna wersja opublikowana
w momencie opublikowania
2023-08-24
70
2,8
0
0